| Rolle | Person |
|---|---|
| Batman / Bruce Wayne | David Nathan |
| Commissioner James Gordon | Wolfgang Wagner |
| Joker | Bodo Wolf |
| Oracle / Barbara Gordon | Gundi Eberhard |
| Rolle | Person |
|---|---|
| Aaron Cash | Karlo Hackenberger |
| Alfred Pennyworth | Dieter B. Gerlach |
| Azrael | Andreas Müller |
| Bane / Birdy Colossimo | Jan Spitzer |
| Black Mask / Roman Sionis | Thomas Schmuckert |
| Calendar Man / Julian Gregory Day | Klaus Sonnenschein |
| Catwoman / Selina Kyle | Heide Domanowski |
| Clayface / Basil Karlo | Kaspar Eichel |
| Commissioner James Gordon im DLC »Harley Quinns Rache« | Jan Spitzer |
| Dr. Hugo Strange | Uli Krohm |
| Dr. Stacey Baker | Victoria Sturm |
| Harley Quinn / Dr. Harleen Quinzel | Rubina Nath |
| Hush / Dr. Thomas Elliot | Raimund Krone |
| Jack Ryder | Bernhard Völger |
| Killer Croc / Waylon Jones | Jörg Hengstler |
| Mad Hatter / Jervis Tetch | Christoph Banken |
| Martha Wayne | Anke Reitzenstein |
| Mr. Freeze / Victor Fries | Olaf Reichmann |
| Pinguin / Oswald Cobblepot | Joachim Kaps |
| Posion Ivy / Dr. Pamela Isley | Christin Marquitan |
| Quincy Sharp | Eberhard Prüter |
| Ra's al Ghul | Kaspar Eichel |
| Riddler / Edward Nigma | Dennis Schmidt-Foß |
| Robin / Tim Drake | Konrad Bösherz |
| Solomon Grundy | Bernd Vollbrecht |
| Two-Face / Harvey Dent | Tim Moeseritz |
| Vicky Vale | Irina von Bentheim |
| Victor Zsasz | Jan-David Rönfeldt |