| Charakter | Person |
|---|---|
| Batman/Bruce Wayne | David Nathan |
| Commissioner James Gordon (Hauptspiel) | Wolfgang Wagner |
| Joker | Bodo Wolf |
| Oracle/Barbara Gordon | Gundi Eberhard |
| Charakter | Person |
|---|---|
| Aaron Cash | Karlo Hackenberger |
| Alfred Pennyworth | Dieter B. Gerlach |
| Azrael | Andreas Müller |
| Bane/Birdy Colossimo | Jan Spitzer |
| Black Mask/Roman Sionis | Thomas Schmuckert |
| Calendar Man/Julian Gregory Day | Klaus Sonnenschein |
| Catwoman/Selina Kyle | Heide Domanowski |
| Clayface/Basil Karlo | Kaspar Eichel |
| Commissioner James Gordon (DLC) | Jan Spitzer |
| Dr. Hugo Strange | Uli Krohm |
| Dr. Stacey Baker | Victoria Sturm |
| Harley Quinn/Dr. Harleen Quinzel | Rubina Nath |
| Hush/Dr. Thomas Elliot | Raimund Krone |
| Jack Ryder | Bernhard Völger |
| Killer Croc/Waylon Jones | Jörg Hengstler |
| Mad Hatter/Jervis Tetch | Christoph Banken |
| Martha Wayne | Anke Reitzenstein |
| Mr. Freeze/Victor Fries | Olaf Reichmann |
| Pinguin/Oswald Cobblepot | Joachim Kaps |
| Posion Ivy/Dr. Pamela Isley | Christin Marquitan |
| Quincy Sharp | Eberhard Prüter |
| Ra's al Ghul | Kaspar Eichel |
| Riddler/Edward Nigma | Dennis Schmidt-Foß |
| Robin/Tim Drake | Konrad Bösherz |
| Solomon Grundy | Bernd Vollbrecht |
| Two-Face/Harvey Dent | Tim Moeseritz |
| Vicky Vale | Irina von Bentheim |
| Victor Zsasz | Jan-David Rönfeldt |
