| Rolle | Person |
|---|---|
| Alfred Pennyworth | Jürgen Thormann |
| Arkham Knight / Jason Todd/Red Hood Red Hood nur DLC | Julien Haggège |
| Batman / Bruce Wayne | David Nathan |
| Joker | Bodo Wolf |
| Oracle / Barbara Gordon / Batgirl Batgirl nur DLC | Tanya Kahana |
| Rolle | Person |
|---|---|
| Azrael / Michael Lane | Jörg Hengstler |
| Black Mask / Roman Sionis | Marco Kröger |
| Blackfire / Joseph Blackfire | Eberhard Haar |
| Catwoman / Selina Kyle | Heide Domanowski |
| Commissioner James Gordon | Jan Spitzer |
| Deathstroke / Slade Wilson | Erich Räuker |
| Firefly / Garfield Lynns | Mario Hassert |
| Francine Langstrom | Giuliana Jakobeit |
| Harley Quinn / Dr. Harleen Quinzel | Rubina Nath |
| Hush / Dr. Thomas Elliot | David Nathan |
| Iron Heights Gefängnisleiter nur DLC | Lutz Mackensy |
| Jack Ryder | Bernhard Völger |
| Killer Croc / Waylon Jones nur DLC | Jörg Hengstler |
| Lucius Fox | Joachim Tennstedt |
| Mad Hatter / Jervis Tetch nur DLC | Christoph Banken |
| Man-Bat / Dr. Kirk Langstrom | Klaus-Peter Grap |
| Mr. Freeze / Victor Fries nur DLC | Olaf Reichmann |
| Nightwing / Dick Grayson | Wanja Gerick |
| Officer Aaron Cash | Michael Iwannek |
| Pinguin / Oswald Cobblepot | Joachim Kaps |
| Poison Ivy / Dr. Pamela Isley | Christin Marquitan |
| Professor Pyg / Lazlo Valentin | Christoph Banken |
| Ra's al Ghul nur DLC | Kaspar Eichel |
| Riddler / Edward Nigma | Dennis Schmidt-Foß |
| Robin / Tim Drake | Konrad Bösherz |
| Scarecrow / Dr. Jonathan Crane | Michael Pan |
| Simon Stagg | Walter Alich |
| Two-Face / Harvey Dent | Tim Moeseritz |
| Vicki Vale | Irina von Bentheim |