| Rolle | Person |
|---|---|
| Harry Osborn | Nils Kreutinger |
| Martin Li / Mister Negative | Florian Hoffmann |
| Mary Jane „MJ“ Watson / Scream | Rieke Werner |
| Miles Morales / Spider-Man | Lars Waltl |
| Peter Parker / Spider-Man | Felix Mayer |
| Sergei Kravinoff / Kraven the Hunter | Johann Fohl |
| Venom | Marios Gavrilis |
| Rolle | Person |
|---|---|
| Aaron Davies / Prowler | ??? |
| Aleksei Sytsevich / Rhino | Christian Jungwirth |
| Angela | Anja Taborsky |
| Betsy Schneider | ??? |
| Bürgermeister Norman Osborn | Kai Henrik Möller |
| Chief Clarke | ??? |
| Cole Wittmann | Rieke Werner |
| Dalisay / Schülerin | Rieke Werner |
| Danika Hart | Melinda Rachfahl |
| Die Flamme / Cletus Kasady | Jean Paul Baeck |
| Direktorin Evans | ??? |
| Dr. Curt Connors / Lizard | ??? |
| Dr. Foster | Tobias Brecklinghaus |
| Dr. Nguyen | ??? |
| Dr. Otto Octavius / Doc Ock | Dirk Hardegen |
| Dr. Young | ??? |
| Eugene | Matthias Hoff |
| Felicia Hardy / Black Cat | Lara „Loft“ Trautmann |
| Flint Marko / Sandman | Peter Sura |
| Ganke | ??? |
| Großer Jäger | ??? |
| Hailey Coopers | Christina Ann Zalamea |
| Howard, der Taubenfütterer | Achim Barrenstein |
| J. Jonah Jameson | Hanns Jörg Krumpholz |
| Jefferson Davis | Michael-Che Koch |
| Kiosk | ??? |
| Lonnie Lincoln / Tombstone | Tobias Brecklinghaus |
| Lucas | ??? |
| MacDonald „Mac“ Gargan / Scorpion | Michael-Che Koch |
| Mr. Atlas | ??? |
| Mr. Sinclair | ??? |
| Ms. Ferguson | ??? |
| Nachahmer-Maskottchen | ??? |
| Odyssey | ??? |
| Quentin Beck / Mysterio | ??? |
| Rio Morales | Gisa Bergmann |
| Robbie Robertson | ??? |
| Sasha Kravinoff | ??? |
| Tante May | ??? |
| Teos-Maskottchen | Jens Wendland |
| Travis Trujillo | Erik Borner |
| Vijay | ??? |
| Wachmann (Schule, Flashback) | ??? |
| Wilson Fisk / Kingpin | Thomas Balou Martin |
| Yuri Watanabe / Wraith | Yesim Meisheit |