| Rolle | Person |
|---|---|
| Desmond Miles | Thomas Friebe |
| Ezio Auditore da Firenze | Markus Pfeiffer |
| Leonardo da Vinci | Simon Jäger |
| Lucy Stillmann | Susanne Dobrusskin |
| Shaun Hastings | Philipp Schepmann |
| Rolle | Person |
|---|---|
| Animus-Stimme | Heike Hagen |
| Brieffälscherin | Inka Lioba Bretschneider |
| Christina Vespucci | Brit Gülland |
| Clay Kaczmarek | Fritz Rott |
| Dr. Warren Vidic | Rainer Delventhal |
| Fabio Orsini | Michael-Che Koch |
| Juan Borgia | Jürg Löw |
| Juno | Claudia Gahrke |
| Lukrezia Borgia | Edda Fischer |
| Maria Auditore da Firenze | Dana Geissler |
| Mario Auditore da Firenze | Helmut Krauss |
| Niccolo Machiavelli | Sebastian Schlemmer |
| Nikolaus Koperniku | Markus Haase |
| Octavien de Valois | Christophe Lascombes |
| Pentisileia d'Alviano | Katja Liebing |
| Rebecca Crane | Michaela Breit |
| Rodrigo Borgia | Wolf Frass |
| William Miles | Bodo Wolf |